The Pineal Gland, Melatonin, & 5-MeO-DMT: The Connection.

One study [1] published in the Journal of Psychopharmacology in 2017 by David E. Nichols showed for the first time that the rat brain produces DMT (dimethyltryptamine) through a biosynthetic reaction catalyzed by the enzymes aromatic-L-amino acid decarboxylase (AADC) and indolethylamine-N-methyltransferase (INMT). The author noted that the same pathway is likely also present in the human (and general mammalian) brain as DMT has been identified endogenously in same. Other than producing about 30 µg/day of melatonin, the pineal gland has been identified with the production of minute amounts of 5-MeO-DMT; an active form of dimethyltryptamine also found in hallucinogenic substances like secretions from the Kambo Toad’s dorsal glands or the root bark of the key plant in the traditional Ayahuasca brew. There are many hundreds of other plant species which contain either DMT, Bufotenin (5-MeO-DMT) , or similarly-structured hallucinogenic compounds. Another study [8] showed that melatonin “can be metabolized into 5-OH-indole-3-acetate in the liver, presumably via serotonin.” Notably the gut microbiome can manufacture as much as 95% of all daily serotonin. This is also why bone broth is so essential as it contains everything we need to maintain nervous and endocrine health which are both so dependent on the gut-brain axis, not to mention the gut-immune axis. Getting offtrack though. 

The metabocard for 5-hydroxy-indole-3-acetate [9] shows that 5-hydroxyindoleacetic acid, as its also known, is involved in the metabolism of tryptophan, which is the molecule from which not only DMT but nearly all hallucinogens and many endocrine compounds are built upon. Dietary tryptophan is the precursor for the synthesis of serotonin, melatonin, DMT, and Bufotenin. It seems then that at least some of these tryptophan metabolites are interchangeable through two-way reactions, and the widely circulated claim about the ‘pitch black room for a week’ This is why it should not be supplemented, same for melatonin and the rest of tryptophan’s metabolites, as they are all so essential for the body that, if the biochemical systems which regulate their production are in disarray, the root source for the imbalance should be addressed (intestinal malabsorption, soft-tissue calcification, blue-light exposure[10], xenobiotics, stress, etc.). For instance, if your melatonin is low, don’t take melatonin; that’s the allopathic, symptomatic, surface-level approach. Instead, take herbal extracts of lavender or any strong verbena species for allowing the body to find and fix its own endocrine or other underlying regulatory mechanism issue, returning into homeostasis, and hence improving sleep.

The pineal gland is not as insignificant as academic curriculums purport it to be, and empirical research is steadily emerging, revealing its deep involvement with several key biological processes through its production of melatonin and DMT alone. The presence of all enzymes needed for the synthesis of di-methyl-tryptamine (DMT) in pineal gland also confirms that it is the source for its production, not merely an active site for it. [11] The various audio-visual hallucinations in near-death-experience phenomena occur due to massive increase of DMT in pineal gland before death. [11] This same study concluded that “a very high concentration of di-methyl-tryptamine (DMT), presence of retinal proteins in 5–10% of pinealocytes, its role in thermoregulation and a possible role as magnetoreceptor in blind men and highest deposits of fluoride in the body are not only interesting but significant for the future research” 

In another extremely intriguing study, volunteers were kept in a pitch-black room for 48 hours, and after a certain amount of time, they began to show strange signs, such as one participant’s account: ”At one point, I started singing and then I burst into tears,” Bloom says. “I can’t remember the last time I cried, and I felt my emotions were beginning to run out of control.” This is possibly due to the excessive melatonin, which could be getting converted to 5-MeO-DMT, as that reaction is likely only favorable at certain very high levels of melatonin. This can also explain the anti-depressant effect that high-dose melatonin supplements are often sought after for. This study gets even more interesting, because “after 40 hours, Bloom began to hallucinate: he saw a pile of 500 oyster shells.” “I could see the pearly sheen on the oyster shells as clear as day,” he says. This is a clear giveaway that whatever endocrine concoction is being produced in his brain due to the lack of any light, stimuli, it has hallucinogenic properties. Given the clear biochemical and structural connection between DMT, melatonin, and serotonin discussed above, it is possible that a mixture of these were involved in producing his experience. 

Also check out the post I did a while ago on fluoride at with over 70 referenced studies on fluoride’s calcifying effect on the pineal gland, coronary arteries, and other soft tissues.

1] Biosynthesis and Extracellular Concentrations of N,N-dimethyltryptamine (DMT) in Mammalian Brain.

2] N,N-dimethyltryptamine and the pineal gland: Separating fact from myth.

3] LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate.

4] N, N-Dimethyltryptamine (DMT), an Endogenous Hallucinogen: Past, Present, and Future Research to Determine Its Role and Function. 

5] Updated View on the Relation of the Pineal Gland to Autism Spectrum Disorders.

6] An investigation of demethylation in the metabolism of methoxytryptamine and methoxytryptophol.

7] Synthesis and Characterization of 5-MeO-DMT Succinate for Clinical Use.

8] 6 Hydroxymelatonin. 

9] Metabocard for 5-Hydroxyindoleacetic acid. 

10] Suppression of Blue Light at Night Ameliorates Metabolic Abnormalities by Controlling Circadian Rhythms. 

11] Pineal gland: A structural and functional enigma